Journal: BMC Neuroscience

Article Title: Chronic exposure to high fat diet triggers myelin disruption and interleukin-33 upregulation in hypothalamus

doi: 10.1186/s12868-019-0516-6

Figure Lengend Snippet: List of antibodies in the study for immunofluorescence or western blot analysis

Article Snippet: Polyclonal goat anti-IL-33 , R and D Systems Cat#AF3626, RRID:AB_884269 , Escherichia coli -derived recombinant mouse IL-33 , 1:200 (IF).

Techniques: Immunofluorescence, Western Blot, Purification, Affinity Chromatography, Recombinant, Derivative Assay

Journal: Experimental and molecular pathology

Article Title: Structural Changes in Hair Follicles and Sebaceous Glands of Hairless Mice Following Exposure to Sulfur Mustard

doi: 10.1016/j.yexmp.2014.03.002

Figure Lengend Snippet: Histological sections prepared 1 and 14 days after air exposure (CTL), and 1, 3, 7 and 14 days after SM exposure were stained with an antibody to FGFR2. Antibody binding was visualized using a Vectastain Elite ABC kit (original magnification, × 400). One representative section from 3 mice/treatment group is shown.

Article Snippet: Tissue sections were incubated at overnight at 4°C with rabbit affinity purified polyclonal antibodies against cleaved caspase-3 (1:200, Cell Signaling, Danvers, MA), fatty acid synthase (1:100, Cell Signaling), fibroblast growth factor receptor 2 (FGFR2) (1:2000, Abcam, Cambridge, MA), phospho-histone 2A.X (Ser139) (phospho-H2A.X) (1:100, Cell Signaling), proliferating cell nuclear antigen (PCNA) (1:250, Abcam), goat affinity purified polyclonal antibody galectin-3 (1:2000, R&D Systems, Minneapolis, MN) or appropriate controls.

Techniques: Staining, Binding Assay